Recombinant Human TLK2/PKU-ALPHA Protein

SKU: PKSH030362

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: HsHPK;PKU-ALPHA

Research Areas: Cell Biology,Epigenetics and Nuclear Signaling

Conjugation:

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Application:

Fusion tag: None

UNIProt ID: Q86UE8

Accession: Q86UE8-1

Background: Serine / threonine-protein kinase tousled-like 2; also known as PKU-alpha; Tousled-like kinase 2 and TLK2; is a nucleus protein which belongs to theprotein kinase superfamily and Ser/Thr protein kinase family. The tousled-like kinases are an evolutionarily conserved family of proteins implicated in DNA repair; DNA replication and mitosis in metazoans and plants. Their absence from the yeasts and other eukaryotic 'microbes' suggests a specific role for them in the development of multicellular organisms. Tousled-like kinase 2 / TLK2 is widely expressed. It is present in fetal placenta; liver; kidney; pancreas; heart and skeletal muscle. It is also found in adult cell lines. Tousled-like kinase 2 / TLK2 contains oneprotein kinase domain. Tousled-like kinase 2 / TLK2 is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.

Concentration:

Activity: Not validated for activity

Sequence: Leu 397-Asn772

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 8.0

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 43.6 kDa

ObservedMW: 44 kDa