Recombinant Human TGFB1/TGF-beta 1 Protein

SKU: PKSH032007

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: TGFB1;TGF-beta 1

Target Synonym: CED;DPD1;LAP;Latency-Associated Peptide;TGF-Beta-1;TGF-beta 1;TGFB;TGFB1;Transforming Growth Factor Beta-1

Research Areas: Signal Transduction;Cell biology;Cardiovascular;Cancer;metabolism;Stem cells

Conjugation:

Target Species: Human

Expression Host: CHO Stable Cells

Application: Cell culture

Fusion tag: None

UNIProt ID: P01137

Accession: P01137

Background: Transforming Growth Factor β-1 (TGFβ-1) is a secreted protein which belongs to the TGF-β family. TGFβ-1 is abundantly expressed in bone; articular cartilage and chondrocytes and is increased in osteoarthritis (OA). TGFβ-1 performs many cellular functions; including the control of cell growth; cell proliferation; cell differentiation and apoptosis. The precursor is cleaved into a latency-associated peptide (LAP) and a mature TGFβ-1 peptide. TGFβ-1 may also form heterodimers with other TGFβ family members. It has been found that TGFβ-1 is frequently upregulated in tumor cells. Mutations in this gene results in Camurati-Engelmann disease.

Concentration:

Activity: Measured by its ability to inhibit the IL-4-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 4-40 pg/ml

Sequence: Ala279-Ser390

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 50mM Glycine-HCl, 150mM NaCl, pH 2.5.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.

Calculated MW: 12.8 kDa

ObservedMW: 13 kDa