Recombinant Human sTNF RII/TNFRSF1B protein

SKU: CD00870

Size:5 ug

Background:Tumor Necrosis Factor Receptor-I (TNF RI, also known as p55/p60) and TNF RII (also known as p75/p80) are both high affinity receptors for TNF-alpha and -beta, potent mediators of multiple aspects of inflammatory immune responses. Both RI and RII are prototypic members of the TNF receptor superfamily and have been designated TNFRSF1A and TNFRSF1B, respectively. Whereas all cell types are thought to express TNF RI, TNF RII expression is limited primarily to hematopoietic cells and cells of the immune system. Most of the biological functions of TNF are mediated via TNF RI. Soluble forms of both TNF RI and TNF RII, generated as a result of proteolytic cleavage of the extracellular domains, have been detected in vivo in various biological fluids. The soluble TNF receptors can bind TNF with high affinity and functions as TNF antagonists.

Research_area:Others

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human soluble Tumor Necrosis Factor Receptor Type II/TNFRSF1B

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 cells is less than .2 μg/ml, corresponding to a specific activity of > 5 IU/mg in the presence of .25 ng/mL of rHuTNF-α.

Synonyms:sTNF RII/TNFRSF1B, CD12b, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR2, TNFR8, p75, p75TNFR,Human

Endotoxin:Less than 0.1 EU/μg of rHusTNF RII/TNFRSF1B as determined by LAL method.

Storage:-20ºC

Sequence:MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT

uniprot accession:P2333

Molecular weight:Approximately 2. kDa, a single non-glycosylated polypeptide chain containing 184 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered solution in PBS, pH 7.4.