Recombinant Human sRANK Receptor protein

SKU: CD00883

Size:20 ug

Background:RANK Ligand and RANK Receptor are members of the TNF superfamily that play an important role in the regulation of specific immunity and bone turnover. RANK Receptor was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate na ve T cell proliferation and to promote the survival of RANK + T cells. RANK is also expressed in a variety of tissues including skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. The RANK/RANKL interaction is important in the regulation of osteoclastogenesis and in dendritic-cell-mediated T cell immune responses. Impairments in RANK signaling have been implicated in the induction of expansile osteolysis and Paget disease of bone (PDB2). Recombinant human sRANK receptor is a 19.1 kDa polypeptide containing the TNFR homologous cysteine rich portion of the extracellular domain of RANK receptor (174 amino acid residues).

Research_area:Immunology

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human soluble Receptor Activator of Nuclear Factor kappa-B Receptor

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by its ability to inhibit sRANK Ligand induced nuclear factor kappa B(NFkappaB) in RAW 264.7 cells is less than 5 ng/ml, corresponding to a specific activity of > 2. × 14 IU/mg in the presence of 15 ng/ml of recombinant sRANK Ligand.

Synonyms:sRANK Receptor, Human

Endotoxin:Less than 0.1 EU/μg of rHusRANK Receptor as determined by LAL method.

Storage:-20ºC

Sequence:QIAPPCTSEK HYEHLGRCCN KCEPGKYMSS KCTTTSDSVC LPCGPDEYLD SWNEEDKCLL HKVCDTGKAL VAVVAGNSTT PRRCACTAGY HWSQDCECCR RNTECAPGLG AQHPLQLNKD TVCKPCLAGY FSDAFSSTDK CRPWTNCTFL GKRVEHHGTE KSDAVCSSSL PARK

uniprot accession:Q9Y6Q6

Molecular weight:Approximately 19.1 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM Tris-HCl, pH 8., 15mM NaCl.