Recombinant Human SIRT3 Protein(Sumo Tag)

SKU: PDEH101116

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:SIRT3

Target Synonym:NAD-dependent deacetylase sirtuin-3;SIR2-like protein 3;SIR2L3

Research Areas:Metabolism;Epigenetics and Nuclear Signaling

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-Sumo

UNIProt ID:Q9NTG7-1

Accession:Q9NTG7-1

Background:SIRT3 is a nicotinamide adenine dinucleotide (NAD(+))-dependent mitochondrial protein deacetylase purported to influence metabolism through post-translational modification of metabolic enzymes. Sirtuin 3 (SIRT3) mediates cellular resistance toward various forms of stress. sirtuin 3 (SIRT3), the mitochondrial NAD(+)-dependent deacetylase, may regulate mitochondrial function and biosynthetic pathways such as glucose and fatty acid metabolism and the tricarboxylic acid (TCA) cycle, oxidative stress, and apoptosis by reversible protein lysine deacetylation. SIRT3 regulates glucose and lipid metabolism and maintains myocardial ATP levels, which protects the heart from metabolic disturbances. SIRT3 can also protect cardiomyocytes from oxidative stress-mediated cell damage and block the development of cardiac hypertrophy. Recent reports show that SIRT3 is involved in the protection of several heart diseases.

Concentration:

Activity:Not validated for activity

Sequence:Gly202-Lys399

Purity:> 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method

Calculated MW:34.5 kDa

ObservedMW:36 kDa