Recombinant Human SGSH Protein (His Tag)

SKU: PKSH032823

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: SGSH

Target Synonym: HSS;N-Sulphoglucosamine Sulphohydrolase;SGSH;Sulfoglucosamine Sulfamidase;Sulphamidase

Research Areas: Signal Transduction;Tags & Cell Markers;Neuroscience;

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: P51688

Accession: P51688

Background: N-Sulphoglucosamine Sulphohydrolase (SGSH) is an important member of the sulfatase family which is involved in the degradation of heparin sulfate. SGSH binds one calcium ion per subunit as a cofactor. SGSH catalyzes N-sulfo-D-glucosamine and H2O to D-glucosamine and sulfate. SGSH deficiency is result in mucopolysaccharidosis type 3A (MPS3A), a recessive lysosomal storage disease characterized by neurological dysfunction but relatively mild somatic manifestations.

Concentration:

Activity: Not validated for activity

Sequence: Arg21-Leu502

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM CaCl₂, 10% Glycerol, pH 7.5.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 55.7 kDa

ObservedMW: 63 kDa