Recombinant Human SDC-4 protein (His Tag)

SKU: CD00954

Size:10 ug

Background:Syndecan-4 (SDC-4) encoded by the SDC-4 gene in humans, has a molecular weight of ~20 kDa. It is one of the four vertebrate syndecans which belong to the syndecan family of Type 1 transmembrane proteins and capable of carrying heparin sulfate (HS) and chondroitin sulfate glycosaminoglycans. Syndecans are the best-characterized plasma membrane proteoglycans with two conserved cytoplasmic domains and divergent extracellular portions, except for HS attachment sites. SDC-4 is the most similar to SDC-2, but is more universally expressed and is found in virtually every cell type. Expression can be upregulated by TGFβ2 and in response to mechanical stress in smooth muscle, wound healing, arterial injury or acute myocardial infarction, probably in response to at least one inflammatory mediator. SDC-4 has more widespread distribution than other syndecans and it is the only syndecan that has been found consistently in focal adhesions. Human SDC-4 ECD shares approximately 79 %, 78 % and 81% a.a. identity with mouse, rat and porcine SDC-4 ECD, respectively.

Research_area:Others

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Syndecan-4

Biological activity:Fully biologically active when compared to standard. The specific activity is determined by binding ability in a functional ELISA. Immobilized rHuSDC-4 at 5 ng/ml (1 μl/well) can bind rHubFGF with a linear range of .1-1 ng/ml.

Synonyms:SDC-4, SYND4, Human

Endotoxin:Less than 0.1 EU/μg of rHuSYND4 as determined by LAL method.

Storage:-20ºC

Sequence:MASSIRETEV IDPQDLLEGR YFSGALPDDE DVVGPGQESD DFELSGSGDL DDLEDSMIGP EVVHPLVPLD NHIPERAGSG SQVPTEPKKL EKNEVIPKRI SPVEESEDVS NKVSMSSTVQ GSNIFERTEV LALEHHHHHH

uniprot accession:P31431

Molecular weight:Approximately 13.9 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids. But it migrates with an apparent molecular mass of 22 kDa in SDS-PAGE.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM PB, pH 7.4.