Recombinant Human sCD40 Ligand protein

SKU: CD00966

Size:10 ug

Background:CD40 ligand is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family and can be cleaved into two chains: the CD40 ligand membrane form and CD40 ligand soluble form. It is primarily expressed on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. It mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation. CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human soluble CD40 Ligand

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-1 ng/ml.

Synonyms:sCD4 Ligand,CD4LG,CD154, CD4L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD4L, Human

Endotoxin:Less than 1 EU/μg of rHusCD40L as determined by LAL method.

Storage:-20ºC

Sequence:MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL

uniprot accession:P29965

Molecular weight:Approximately 16.3 kDa, a single non-glycosylated polypeptide chain containing 149 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7..