Recombinant Human RSPO3 Protein (aa 1-146, His Tag)

SKU: PKSH031223

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: CRISTIN1;PWTSR;Protein with TSP type-1 repeat;R-spondin-3;RSPO3;Roof plate-specific spondin-3;THSD2;Thrombospondin type-1 domain-containing protein 2

Research Areas: Stem Cells,Developmental Biology

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-His

UNIProt ID: Q9BXY4

Accession: Q9BXY4-1

Background: R-spondin 3 (RSPO3) is a member of the R-Spondin (RSPO) family in vertebrates that activate Wnt/beta-catenin signaling; plays a key role in these processes. The RSPO family of secreted Wnt modulators is involved in development and disease and holds therapeutic promise as stem cell growth factors. The four members have high structural homology. RSPO2 and RSPO3 are more potent than RSPO1; whereas RSPO4 is relatively inactive. All RSPO members require Wnt ligands and LRP6 for activity and amplify signaling of Wnt3A; Wnt1; and Wnt7A; suggesting that RSPO proteins are general regulators of canonical Wnt signaling. RSPO3/PCP signaling during gastrulation requires Wnt5a and is transduced via Fz7; Dvl; and JNK. RSPO3 functions by inducing Sdc4-dependent; clathrin-mediated endocytosis. RSPO3 is a novel; evolutionarily conserved angiogenic factor in embryogenesis. RSPO3 has a key role in the interaction between chorion and allantois in labyrinthine development.

Concentration:

Activity: Immobilized RSPO3-His (146) at 10 μg/mL (100 μL/well) can bind human RNF43-Fc. The EC50 of human RNF43-Fc is 0.01-0.03μg/mL.

Sequence: Met 1-Val 146

Purity: > 96 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 15.3 kDa

ObservedMW: