Recombinant Human Ribonuclease A/RNASE1 Protein (His Tag)

SKU: PKSH033004

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: Ribonuclease A;RNASE1

Target Synonym: HP-Rnase;RIB-1;RIB1;RNASE1;RNS1;RNase 1;RNase A;RNase UpI-1;Ribonuclease 1;Ribonuclease A;Ribonuclease Pancreatic

Research Areas: epigenetics and nuclear signal

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: P07998

Accession: P07998

Background: Ribonuclease Pancreatic is a secreted enzyme that belongs to the pancreatic ribonuclease family. RNASE1 is an endonuclease that cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. RNASE1 prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. RNASE1 acts on single stranded and double stranded RNA.

Concentration:

Activity: Not validated for activity

Sequence: Lys29-Thr156

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 10% Glycerol, pH 7.4.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 15.6 kDa

ObservedMW: 17-33 kDa