Recombinant Human RANTES protein (GST&His tag)

SKU: PDEH100876

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:RANTES

Target Synonym:C-C Motif Chemokine 5;CCL5;D17S136E;EoCP;Eosinophil Chemotactic Cytokine;SCYA5;SIS-Delta;Small-Inducible Cytokine A5;T Cell-Specific Protein P228;T-Cell-Specific Protein RANTES;TCP228

Research Areas:Cardiovascular;immunology

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-GST & C-His

UNIProt ID:P13501

Accession:P13501

Background:Human Chemokine (C-C Motif) Ligand 5 (CCL5) plays an active role in recruiting leukocytes into inflammatory sites. CCL5 is secreted by many cell types at inflammatory sites and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5. N-Terminal truncated CCL5/RANTES, Met-RANTES, and amino-oxypentane (AOP)-RANTES exhibit antagonist or partial agonist functions on their receptors. CCL5/RANTES attracts different subtypes of leukocytes into inflamed tissue and intervenes in a wide range of allergic and autoimmune diseases.

Concentration:

Activity:Not validated for activity

Sequence:Pro 25-Ser 91

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:32.3 kDa

ObservedMW:42 kDa