Recombinant Human PRKD2/PKD2 Protein (His & GST Tag)

SKU: PKSH030353

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: HSPC187;PKD2;nPKC-D2

Research Areas: Signal Transduction;

Conjugation:

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Application: Enzyme

Fusion tag: N-His-GST

UNIProt ID: Q9BZL6

Accession: NP_057541.2

Background: Serine/threonine-protein kinase D2, also known as PRKD2 and PKD2, is a cytoplasm and membrane protein which belongs to theprotein kinase superfamily, CAMK Ser/Thr protein kinase family and PKD subfamily. PRKD2 / PKD2 is widely expressed. It contains onePH domain, twophorbol-ester/DAG-type zinc fingers and oneprotein kinase domain. PRKD2 / PKD2 is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-710 and phosphorylation of Ser-706 by PKC relieves auto-inhibition by the PH domain. PRKD2 / PKD2 converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.

Concentration:

Activity: The specific activity was determined to be > 30 nmol/min/mg using synthetic CREBtide peptide (KRREILSRRPSYR) as substrate.

Sequence: Met 1-Leu 878

Purity: > 82 % as determined by reducing SDS-PAGE.

Formulation: Supplied as sterile solution of 50mM Tris, 500mM NaCl, 0.5mM PMSF, 10% glycerol, pH 8.0

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 124 kDa

ObservedMW: 120 kDa