Recombinant Human PIK3R5 protein (His tag)

SKU: PDEH100963

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:PIK3R5

Target Synonym:PIK3R5;PI3-kinase regulatory subunit 5;Phosphoinositide 3-kinase regulatory subunit 5

Research Areas:Signal Transduction

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-His & C-His

UNIProt ID:Q8WYR1

Accession:Q8WYR1-2

Background:Phosphoinositide 3-kinase regulatory subunit 5 (PIK3R5, also PI3-kinase p101 subunit, Ptdlns-3-kinase p101, and p101-PI3K) is a 97 kDa regulatory subunit of the PI3K gamma complex. It is expressed as a heterodimer with the catalytic subunit PIK3CG/p120. Human PIK3R5 is 880 amino acids (aa) in length. The heterodimerization region is made up of aa 25-101, and aa 653-753 comprise the region for interaction with G beta gamma proteins. A second 55 kDa isoform is formed by the deletion of aa 1-386.

Concentration:

Activity:Not validated for activity

Sequence:Met 1-Pro 494

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:54.2 kDa

ObservedMW:58 kDa