Recombinant Human PDGF-BB Protein

SKU: PKSH032906

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: PDGF-BB

Target Synonym: Becaplermin;PDGF Subunit B;PDGF-2;PDGF2;PDGFB;Platelet-Derived Growth Factor B Chain;Platelet-Derived Growth Factor Beta Polypeptide;Platelet-Derived Growth Factor Subunit B;Proto-Oncogene c-Sis;SIS

Research Areas: Signal Transduction;Cardiovascular;Cancer;Developmental Biology;metabolism;

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Cell culture

Fusion tag: None

UNIProt ID: P01127

Accession: P01127

Background: Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF/VEGF growth factor family. Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.Binding of PDGFB to its receptor elicits a variety of cellular responses. In addition, PDGFB is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.

Concentration:

Activity: Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED₅₀ for this effect is 5-20 ng/ml.

Sequence: Ser82-Thr190

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM NaAc-HAc, pH 4.5.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.2 EU per μg of the protein as determined by the LAL method.

Calculated MW: 12.4 kDa

ObservedMW: 14 kDa