Recombinant Human PDGF-AA Protein

SKU: PKSH032904

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: PDGF-AA

Target Synonym: PDGF subunit A;PDGF-1;PDGF1;PDGFA;Platelet-derived growth factor A chain;Platelet-derived growth factor alpha polypeptide;Platelet-derived growth factor subunit A

Research Areas: Signal Transduction;Cardiovascular;Cancer;Developmental Biology;immunology;metabolism;

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: None

UNIProt ID: P04085

Accession: P04085

Background: Platelet-derived growth factor subunit A (PDGFA); belongs to the PDGF/VEGF growth factor family. PDGFA is a secreted protein; stored in platelet alpha-granules and released by platelets upon wounding. PDGFA is potent mitogens for a variety of cell types including smooth muscle cells; connective tissue cells; bone and cartilage cells; and some blood cells. It plays an essential role in the regulation of embryonic development; cell proliferation; cell migration; survival and chemotaxis. PDGFA is required for normal lung alveolar septum formation during embryogenesis; normal development of the gastrointestinal tract; normal development of Leydig cells and spermatogenesis; normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. It plays an important role in wound healing; Signaling is modulated by the formation of heterodimers with PDGFB.

Concentration:

Activity: Not validated for activity

Sequence: Ser87-Thr 211

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Glycine-HCl, 6% Sucrose, 4% Mannitol, 0.02% Tween 80, pH 3.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 14.4 kDa

ObservedMW: 16 kDa