Recombinant Human PCNA protein (His tag)

SKU: PDEH100932

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:PCNA

Target Synonym:ATLD2;Cyclin;DNA polymerase delta auxiliary protein;Mutagen-sensitive 209 protein;Polymerase delta accessory protein;Proliferating cell nuclear antigen;cb16

Research Areas:Cell Biology;Epigenetics and Nuclear Signaling;Tags & Cell Markers;Cancer;Isotype/Loading Controls

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-His

UNIProt ID:P12004

Accession:P12004

Background:This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.

Concentration:

Activity:Not validated for activity

Sequence:Met 1-Ser 261

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:28.6 kDa

ObservedMW:38 kDa