Recombinant Human Noggin protein

SKU: CD01208

Size:5 ug

Background:Noggin encoded by the NOG gene, was first isolated from Xenopus, having the function of inducing secondary axis formation in frog embryos. It inhibits TGF-β family ligands and preventing them from binding to their corresponding receptors. Noggin was originally found as a BMP-4 antagonist, and then has been shown to modulate the activities of other BMPs (BMP-2, 7, 13 and 14). Additionally, it has pleiotropic effect, both in early development and later stages. The results of the mouse knockout of noggin suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. In recent report, proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) have relation with the mutant of evolutionarily conserved amino acid residues of Noggin. Mature human Noggin shares 99 %, 99 %, 98 %, 97 % and 89 % a.a. sequence identity with mouse, rat, bovine, equine and chicken Noggin, respectively.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Noggin

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by inhibiting BMP-4-induced alkaline phosphatase production of Mouse ATDC5 cells is less than 3. ng/ml, corresponding to a specific activity of > 3.3 × 15 IU/mg in the presence of 5 ng/ml rHuBMP-4.

Synonyms:Nog,Noggin,SYM1, SYNS1, SYNS1A, Human

Endotoxin:Less than 1 EU/μg of rHuNoggin as determined by LAL method.

Storage:-20ºC

Sequence:MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGAAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC

uniprot accession:Q13253

Molecular weight:Approximately 46.3 kDa, non-disulfide-linked homodimer consisting of two 26 amino acid polypeptide chains.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 3 % acetonitrile, .1 % TFA.