Recombinant Human NACHRA5/CHRNA5 Protein (His Tag)

SKU: PKSH033582

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: NACHRA5;CHRNA5

Target Synonym: CHRNA5;NACHRA5;Neuronal Acetylcholine Receptor Subunit Alpha-5

Research Areas: Neuroscience

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: P30532

Accession: P30532

Background: Neuronal Acetylcholine Receptor Subunit α-5 (NACHRA5) is a member of the ligand-gated ion channel family. Neuronal AChR is composed of two different type of subunits: α and non-α. When NACHRA5 binds to acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits, leading to the opening of an ion-conducting channel across the plasma membrane. Genetic variations in NACHRA5 have been related to susceptibility to smoking-related behavioral traits and lung cancer, contributing to the smoking quantitative trait locus 3.

Concentration:

Activity: Not validated for activity

Sequence: Arg23-Thr254

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 27.6 kDa

ObservedMW: 35-47 kDa