Recombinant Human MIP-1 Beta/CCL4 protein

SKU: CD01281

Size:2 ug

Background:CCL4 encoded by the CCL4 gene, also known as Macrophage Inflammatory Protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. It is a monokine with inflammatory and chemokinetic properties. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Specifically, MIP-1-beta (3-69) is also a ligand for CCR1 and CCR2 isoform B. Recombinant human CCL4 contains 69 amino acids and it shares 77 % and 80 % a.a. sequence identity with Mouse and rat CCL4, respectively. Both human and Mouse MIP-1α and MIP-1β are active on human and Mouse hematopoietic cells.

Research_area:Immunology

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Macrophage Inflammatory Protein-1 beta/CCL4

Biological activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 5.-2 ng/ml.

Synonyms:MIP-1β/CCL4, ACT2, AT744.1, G-26, HC21, LAG-1, LAG1, MIP-1-beta, MIP1B, MIP1B1, SCYA2, SCYA4,Human

Endotoxin:Less than 1 EU/ug of rHuMIP-1β/CCL4 as determined by LAL method.

Storage:-20ºC

Sequence:APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRSKQV CADPSESWVQ EYVYDLELN

uniprot accession:P13236

Molecular weight:Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM PB, pH 7.4, 15 mM NaCl.