Recombinant Human metapneumovirus Major surface glycoprotein G (G), partial

SKU: Q6WB94

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Others

Uniprot ID: Q6WB94

Gene Names: G

Alternative Name(s): Attachment glycoprotein G;Membrane-bound glycoprotein;mG

Abbreviation: Recombinant Human metapneumovirus Major surface glycoprotein G, partial

Organism: Human metapneumovirus (strain CAN97-83) (HMPV)

Source: Mammalian cell

Expression Region: 52-219aa

Protein Length: Partial

Tag Info: C-terminal 10xHis-tagged

Target Protein Sequence: NYTIQKTSSESEHHTSSPPTESNKEASTISTDNPDINPNSQHPTQQSTENPTLNPAASVSPSETEPASTPDTTNRLSSVDRSTAQPSESRTKTKPTVHTRNNPSTASSTQSPPRATTKAIRRATTFRMSSTGKRPTTTSVQSDSSTTTQNHEETGSANPQASVSTMQN

MW: 21.0 kDa

Purity: Greater than 95% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Attaches the virion to the host cell membrane by interacting with glycosaminoglycans, initiating the infection. Unlike other paramyxovirus attachment proteins, lacks both neuraminidase and hemagglutinating activities. In addition to its role in attachment, glycoprotein G interacts with host DDX58 and inhibits DDX58-mediated signaling pathway in order to prevent the establishment of the antiviral state.

Reference:

Function: