Recombinant Human Interleukin-18/IL-18 Protein

SKU: PKSH033626

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: Interleukin-18;IL-18

Target Synonym: MGC;IL1F;IL-1F;IL18;IGIF;IL-18;IL-1g;IL1F4;GIF;Iboctadekin;IFN-gamma-inducing factor;IL-1 gamma;IL-1F4;Interferon gamma-inducing factor;Interferon-gamma-inducing factor;Interleukin 18;Interleukin-1 gamma;Interleukin-18;MGC12320;IFN gamma inducing factor;IL 1 gamma;IL 18;IL 1g;IL1 gamma;IL18 protein;IL1g;IL1gamma;ILIF4;Interferon gamma inducing factor;Interleukin 1 gamma;Interleukin 18 (interferon-gamma-inducing factor);Interleukin18

Research Areas: Cancer;immunology;metabolism

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Cell culture

Fusion tag: None

UNIProt ID: Q14116

Accession: Q14116

Background: Interleukin-18 is a secreted protein and it belongs to the IL-1 family. IL-18 is a proinflammatory cytokine and produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL-4 dependent IgE and IgG1 production; and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine; and thus negatively regulate its biological activity. After stimulation with IL-18; natural killer (NK) cells and certain T cells release another important cytokine called interferon-γ (IFN-γ) or type II interferon that plays an important role in activating the macrophages or other cells.

Concentration:

Activity: Measure by its ability to induce IFN gamma secretion in KG-1 cells. The ED₅₀ for this effect is <6 ng/mL.

Sequence: Tyr37-Asp193

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 18.2 kDa

ObservedMW: 15 kDa