GeneBio Systems
Recombinant Human IL-4 (E. coli)
Recombinant Human IL-4 (E. coli)
SKU:EPT139
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Size:50ug
Description:Recombinant Human Interleukin-4 is produced by our E.coli expression system and the target gene encoding His25-Ser153 is expressed.
Accession:P05112
Molecular weight:15.1 KDa
Apparent molecular weight:13-14 KDa, reducing conditions
Other names:Interleukin-4; IL-4; B-Cell Stimulatory Factor 1; BSF-1; Binetrakin; Lymphocyte Stimulatory Factor 1; Pitrakinra; IL4
Storage condition:Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity:Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin:Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
Biological activity:Loaded Cynomolgus IL-4RA-Fc(Cat#C939) on Protein A Biosensor, can bind Human IL-4(Cat#C050) with an affinity constant of <10-3 nM as determined in BLI assay.
Redissolve:Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage:Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Delivery condition:The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Background:Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.
