Recombinant Human IL-36 Receptor Antagonist Protein/IL-36RN/IL-1F5

SKU: PKSH033872

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: IL-36RN;IL-1F5

Target Synonym: FIL;UNQ;PRO;IL1F;IL1L;IL1HY;IL1RP;PSORS;IL36RN;FIL1;FIL1(DELTA);FIL1D;IL-36Ra;IL1F5;IL1HY1;IL1L1;IL1RP3;IL36RA;PSORP;PSORS14;UNQ1896;PRO4342;FIL1 Delta;IL-1 Delta;IL-1F5;IL-1HY1;IL-1L1;IL-1ra Homolog 1;IL-1-Related Protein 3;IL-1RP3;Interleukin-1 Delta;Interleukin-1 Family Member 5;Interleukin-1 HY1;Interleukin-1 Receptor Antagonist Homolog 1;Interleukin-1-Like Protein 1;Interleukin-36 Receptor Antagonist Protein

Research Areas: immunology

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Cell culture

Fusion tag: None

UNIProt ID: Q9UBH0

Accession: Q9UBH0

Background: Human Interleukin-36 Receptor Antagonist (IL-36RN) is a secreted protein which belongs to the Interleukin 1 cytokine family (IL-1 family). IL-36RN is predominantly expressed in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. IL-36RN is also detected in the spleen, brain leukocyte and macrophage cell types. Increased in lesional psoriasis skin. IL-36RN is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to Interleukin 1 family member 9 (IL1F9). Dysregulated expression of novel agonistic and antagonistic IL-1 family member ligands can promote cutaneous inflammation, revealing potential novel targets for the treatment of inflammatory skin disorders. Human and mouse IL-36RN share 90% sequence identity.

Concentration:

Activity: Measured by its ability to inhibit IL-36 alpha, IL-36 beta or IL-36 gamma-induced IL-8 secretion in A431 human epithelial carcinoma cells. The ED₅₀ for this effect is 23.14 ng/ml.

Sequence: Val2-Asp155

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 16.8 kDa

ObservedMW: 17 kDa