Recombinant Human IL-17 protein

SKU: CD01557

Size:5 ug

Background:Human Interleukin-17A (IL-17A) is encoded by the IL17A gene located on the chromosome 6 and belongs to the IL-17 family that contains IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. They have a similar protein structure, with four highly conserved cysteine residues critical to their 3-dimensional shape, but no sequence similarity to any other known cytokines. Interleukin 17 is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. Mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, IL-17 exhibits 63 % amino acid identity with mouse IL-17. High levels of human IL-17 were induced from primary peripheral blood CD4+ T cells upon stimulation and they can induce stromal cells to produce proinflammatory and hematopoietic cytokines.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Interleukin-17

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by inducing IL-6 secretion of Mouse NIH/3T3 cells is less than 7.5 ng/ml, corresponding to a specific activity of > 1.3 × 15 IU/mg.

Synonyms:IL-17,CTLA-8, CTLA8, IL-17, IL17A, IL17, ILA17, Human

Endotoxin:Less than 1 EU/μg of rHuIL-17 as determined by LAL method.

Storage:-20ºC

Sequence:GITIPRNPGC PNSEDKNFPR TVMVNLNIHN RNTNTNPKRS SDYYNRSTSP WNLHRNEDPE RYPSVIWEAK CRHLGCINAD GNVDYHMNSV PIQQEILVLR REPPHCPNSF RLEKILVSVG CTCVTPIVHH VA

uniprot accession:Q16552

Molecular weight:Approximately 31. kDa, a disulfide-linked homodimer of two 132 amino acid polypeptide chains.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.