Recombinant Human IFN-Gamma protein

SKU: CD01599

Size:20 ug

Background:Interferon-gamma (IFN-γ), also known as Type II interferon or immune interferon, is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. Human IFN-γ is highly species specific and is biologically active only in human and primate cells. IFN-γ was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-γ also augments TH1 cell expansion and may be required for TH1 cell differentiation.

Research_area:Immunology

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Interferon-gamma

Biological activity:Fully biologically active when compared to standard. The ED5 as measured in anti-viral assays using human HeLa cells infected with encephalomyocarditis (EMC) virus is .15-.8 ng/ml.

Synonyms:IFN-γ,IFG, IFI, IMD69,IFNG, Human

Endotoxin:Less than 1 EU/μg of rHuIFN-γ as determined by LAL method.

Storage:-20ºC

Sequence:MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRGR RASQ

uniprot accession:P1579

Molecular weight:Approximately 16.9 kDa, a single non-glycosylated polypeptide chain containing 144 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.