Recombinant Human HO1 Protein(Trx Tag)

SKU: PDEH100474

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:HO1

Target Synonym:HSP;heme oxygenase;heme oxygenase (decycling);bK286B;HO-1 HMOX;HMOX1;HMOX1D;HO-1;HSP32;bK286B10;32 kD;D8Wsu38e;heat shock protein 32 kD;heat shock protein 32kD;Hemox;Hmox;HMOX 1;HO 1;HO-1 HMOX1;heme oxygenase (decycling) 1;heme oxygenase 1;heat shock protein;32-kD;HO;HO1;bK286B10;heme oxygenase 1;HMOX1D;HO-1;HSP32

Research Areas:Immunology;Metabolism;Neuroscience;Cardiovascular;Signal Transduction

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-Trx

UNIProt ID:P09601

Accession:P09601

Background:Heme Oxygenase 1 (HO-1) is an enzyme in endoplasmic reticulum that belongs to the heme oxygenase family. HO-1 cleaves the heme ring at the alpha methene bridge to form Biliverdin. Biliverdin is subsequently converted to Bilirubin by Biliverdin reductase. In physiological state, the highest activity of HO-1 is found in the spleen, where senescent erythrocytes are sequestrated and destroyed. HO-1 activity is highly inducible by its substrate heme and by various non-heme substances such as heavy metals, bromobenzene, endotoxin, oxidizing agents and UVA. HO-1 is involved in the regulation of cardiovascular function and response to a variety of stressors. Defects in HO-1 are the cause of Heme Oxygenase 1 deficiency, resulting in marked erythrocyte fragmentation and intravascular hemolysis, coagulation abnormalities, endothelial damage, and iron deposition in renal and hepatic tissues.

Concentration:

Activity:Not validated for activity

Sequence:Met1-Thr261

Purity:> 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method

Calculated MW:48.7 kDa

ObservedMW:48 kDa