Recombinant Human HIP2/UBE2K Protein (His Tag, SUMO Tag)

SKU: PKSH033186

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: HIP2;UBE2K

Target Synonym: HIP-2;HIP2;Huntingtin-Interacting Protein 2;LIG;UBE2K;Ubiquitin Carrier Protein;Ubiquitin-Conjugating Enzyme E2 K;Ubiquitin-Conjugating Enzyme E2(25K);Ubiquitin-Conjugating Enzyme E2-25 kDa;Ubiquitin-Conjugating Enzyme E2-25K;Ubiquitin-Protein Ligase

Research Areas: Cell biology;Neuroscience;epigenetics and nuclear signal

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: N-His-SUMO

UNIProt ID: P61086

Accession: P61086

Background: Ubiquitin-Conjugating Enzyme E2 K (UBE2K) belongs to the E2 Ubiquitin-Conjugating Enzyme family. UBE2K is highly expressed in the brain; with highest levels found in cortex and striatum; and at lower levels in cerebellum and brainstem. UBE2K may mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. UBE2K is associated with the selective degradation of short-lived and abnormal proteins; such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. In addition; UBE2K is involved in Alzheimer's disease; Huntington's disease and antigen processing through its interaction with huntingtin; and MHC-heavy chain proteins.

Concentration:

Activity: Not validated for activity

Sequence: Met 1-Asn200

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 8% Sucrose, 100mM NaCl, 0.05% Tween 80, pH 7.5.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 34.5 kDa

ObservedMW: 38 kDa