Recombinant Human G-CSF/CSF3 Protein (Fc Tag)

SKU: PKSH031938

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: C17orf33;CSF3;Filgrastim;G-CSF;GCSF;Granulocyte Colony-Stimulating Factor;Lenograstim;Pluripoietin

Research Areas: Cancer;immunology

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: Cell culture

Fusion tag: N-hFc

UNIProt ID: P09919

Accession: NP_757373.1

Background: Granulocyte-colony stimulating factor (G-CSF) is a growth factor and an essential cytokine belonging to the CSF family of hormone-like glycoproteins. It is produced by numerous cell types including immune and endothelial cells. Recent animal studies have also revealed that G-CSF activates multiple signaling pathways, such as Akt and also the Janus family kinase-2 and signal transducer and activation of transcription-3 (Jak2-STAT3) pathway, thereby promoting survival, proliferation, differentiation and mobilisation of haematopoietic stem and progenitor cells. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem cell mobilizer and erythropoietin as a cytoprotective agent. G-CSF prevents left ventricular remodeling after myocardial infarction by decreasing cardiomyocyte death and by increasing the number of blood vessels, suggesting the importance of direct actions of G-CSF on the myocardium rather than through mobilization and differentiation of stem cells. Accordingly, recombinant human (rh)G-CSF has been extensively used in clinical haematology and oncology to enable bone marrow transplantation or to treat chemotherapy-associated neutropenia. In preclinical study, G-CSF improved cardiac function and perfusion by angiomyogenesis and protection of cardiomyocytes in myocardial infarction.

Concentration:

Activity: Measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.The ED50 for this effect is typically 0.2-0.8 ng/ml.

Sequence: Ala 30-Pro 204

Purity: > 97 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 45.4 kDa

ObservedMW: 48 kDa