Recombinant Human FGF-19 protein

SKU: CD01806

Size:5 ug

Background:Human FGF-19 is encoded by the FGF19 gene. FGF-19 belongs to the FGF-19 subfamily which has three members FGF-19, 21, 23. FGFs are classically considered to be paracrine factors and are known for their roles in tissue patterning and organogenesis during embryogenesis. By contrast, the FGF-19 subfamily has recently been shown to function in an endocrine manner. Members of this subfamily have poor ability of binding to heparin binding site which is a crucial factor in ligand-receptor complex formation. β-Klotho has been identified as co-factor required for FGF-19, 21, 23 signaling. It can obviously increase ligand-receptor affinity. Unlike most FGFs which bind to and activate more than one FGF receptor, FGF19 is a specific ligand for FGF R4. In FGF-19 transgenic mice, reducing liver triglycerides, increasing fatty acid oxidation, reducing glucose levels and improving insulin sensitivity can be observed.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Fibroblast Growth Factor-19

Biological activity:Assay #1: Fully biologically active when compared to standard. The biological activity is measured by its binding ability in a functional ELISA. Immobilized rHuFGF R4 at 5 μg/ml can bind rHuFGF-19 with a linear range of 3-2 ng/ml. Assay #2: Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse Balb/c 3T3 cells is less than 15 ng/ml, corresponding to a specific activity of > 6.7 × 13 IU/mg.

Synonyms:FGF-19, FGF19,Human

Endotoxin:Less than 1 EU/μg of rHuFGF-19 as determined by LAL method.

Storage:-20ºC

Sequence:MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK

uniprot accession:O9575

Molecular weight:Approximately 21.8 kDa, a single non-glycosylated polypeptide chain containing 195 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.