Recombinant Human Endostatin protein

SKU: CD01870

Size:20 ug

Background:Endostatin has been identified as a C-terminal fragment of Collagen type 18, a recently identified member of a family of collagen-like proteins referred to as multiplexin family. Endostatin specifically inhibits proliferation of endothelial cells although it does not affect the proliferation of EOMA cells. Endostatin also potently inhibits angiogenesis and tumor growth. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes. Recombinant Endosatin expressed in yeast causes G1 arrest of endothelial cells, and endostatin treatment results in apoptosis of HUVE and HMVE cells.

Research_area:Immunology

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Pichia Pastoris

Full product name:Recombinant Human Endostatin

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by inhibiting the FGF basic-dependent proliferation of HUVEC migration is less than 2. μg/mL in the presence of Anti-Human Endostatin Polyclonal Antibody.

Synonyms:Endostatin, Human

Endotoxin:Less than 1 EU/μg of rHuEndostatin as determined by LAL method.

Storage:-20ºC

Sequence:NYEKPALHLA ALNMPFSGDI RADFQCFKQA RAAGLLSTYR AFLSSHLQDL STIVRKAERY SLPIVNLKGQ VLFNNWDSIF SGHGGQFNMH IPIYSFDGRD IMTDPSWPQK VIWHGSSPHG VRLVDNYCEA WRTADTAVTG LASPLSTGKI LDQKAYSCAN RLIVLCIENS FMTDARK

uniprot accession:P396

Molecular weight:Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 177 amino acids (C-terminal fragment of collagen XVIII).

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.