Recombinant Human CXCL2 Protein(Trx Tag)

SKU: PDEH100573

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:CXCL2

Target Synonym:GRO;MIP;CXCL;SCYB;C-X-C Motif Chemokine;CXCL2;CINC-2a;GRO2;GROb;MGSA-b;MIP-2a;MIP2;MIP2A;SCYB2;CINC2a;C-X-C Motif Chemokine 2;GRO beta;Gro-Beta;Growth-Regulated Protein Beta;Macrophage Inflammatory Protein 2-Alpha;MIP2-Alpha

Research Areas:Immunology

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-Trx

UNIProt ID:P19875

Accession:P19875

Background:Chemokine (C-X-C motif) ligand 2 (CXCL2) , also called macrophage inflammatory protein 2 (MIP-2) , Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2) , is a small cytokine belonging to the CXC chemokine family. CXCL2/MIP-2 is selectively up-regulated in tolerance-conferring APCs and serves to recruit NKT cells to the splenic marginal zone , where they form clusters with APCs and T cells. In the absence of the high-affinity receptor for CXCL2/MIP-2 or in the presence of a blocking Ab to CXCL2/MIP-2 , peripheral tolerance is prevented , and Ag-specific T regulatory cells are not generated. CXCL2/MIP-2 is selectively up-regulated in tolerance-conferring APCs and serves to recruit NKT cells to the splenic marginal zone , where they form clusters with APCs and T cells. In the absence of the high-affinity receptor for MIP-2 (as in CXCR2-deficient mice) or in the presence of a blocking Ab to MIP-2 , peripheral tolerance is prevented , and Ag-specific T regulatory cells are not generated. Understanding the regulation of lymphocyte traffic during tolerance induction may lead to novel therapies for autoimmunity , graft acceptance , and tumor rejection. Several studies have implicated the CXCL2 chemokine as a mediator in the development of sepsis. CXCL2/MIP-2 also plays a major role in mediating the neutrophilic inflammatory response of the rodent lung to particles such as quartz , crocidolite asbestos , as well as high doses of other relative innocuous dusts such as titanium dioxide.

Concentration:

Activity:Not validated for activity

Sequence:Ala35-Asn107

Purity: > 90% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:27.9 kDa

ObservedMW:30 kDa