Recombinant Human CXCL12 (24-88) protein(His Tag)

SKU: PKSH034169

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: CXCL12

Target Synonym: IRH;PBSF;SCYB12;SDF1;TLSF;TPAR1

Research Areas: Immunology,Microbiology,Cardiovascular

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: P48061

Accession: P48061

Background: Stromal Cell-Derived Factor-1 (SDF-1) is a chemokine member of the intercrine family. SDF1 is expressed as five isoforms that differ only in the C terminal tail. SDF1α and SDF1β are identical except for the four residues present in the C-terminus of SDF1β but absent from SDF1α. SDF1 isoforms interact with CXCR4 and CXCR7 receptors on the cell surface; and can also bind syndecan4. SDF1 is known to influence lymphopoiesis; regulate patterning and cell number of neural progenitors; and promote angiogenesis. It also enhances the survival of myeloid progenitor cells.

Concentration:

Activity: Measure by its ability to chemoattract BaF3 cells transfected with human CXCR4.The ED₅₀ for this effect is <0.5 ng/mL.

Sequence: Val 24-Leu 195

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20 mM sodium citrate, 0.1 M NaCl, pH 4.5.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 8.6 kDa

ObservedMW: 10 kDa