Recombinant Human Claudin-18.2 (CLDN18.2)-Detergent (Active)

SKU: P56856-2

Size: 100ug. Other sizes are also available.

Activity: Yes

Research Areas: Signal Transduction

Uniprot ID: P56856-2

Gene Names: CLDN18.2

Alternative Name(s): (CLDN18.2)

Abbreviation: Recombinant Human CLDN18.2 protein-Detergent (Active)

Organism: Homo sapiens (Human)

Source: Mammalian cell

Expression Region: 1-261aa

Protein Length: Full Length of Isoform A2

Tag Info: C-terminal 10xHis-tagged

Target Protein Sequence: MAVTACQGLGFVVSLIGIAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV

MW: 29.1 kDa

Purity: Greater than 80% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN18.2 at 5 μg/mL on an Nickel Coated plate can bind Anti-CLDN18.2 recombinant antibody (CSB-RA005505MA1HU), the EC50 is 0.4640-0.6372 ng/mL.

Form: Liquid

Buffer: 50 mM HEPES, 0.15 M NaCl, 0.05% DDM, 0.01% CHS, pH7.5

Reconstitution: /

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

Reference: "Claudin-18 splice variant 2 is a pan-cancer target suitable for therapeutic antibody development." Sahin U., Koslowski M., Dhaene K., Usener D., Brandenburg G., Seitz G., Huber C., Tuereci O. Clin. Cancer Res. 14: 7624-7634(2008)

Function: