Recombinant Human CDCP1/CD318 Protein (aa 30-341, His Tag)

SKU: PKSH033302

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: CDCP1;CD318

Target Synonym: CD318;CUB domain-containing protein 1;Membrane glycoprotein gp140;SIMA135;Subtractive immunization M plus HEp3-associated 135 kDa protein;TRASK;Transmembrane and associated with src kinases

Research Areas: Tags & Cell Markers,Stem Cells,Cancer

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: Q9H5V8

Accession: Q9H5V8-3

Background: CUB domain-containing protein 1(CDCP1) is a transmembrane glycoprotein with a large extracellular domain (ECD) containing two CUB domains; and a smaller intracellular domain (ICD) containing five tyrosines. CDCP1 is widely expressed in human epithelial tissues; but its phosphorylation is only seen in mitotically detached or shedding cells; consistent with its role in the negative regulation of cell adhesion. The tyrosine phosphorylation of CDCP1 in cultured cells occurs when cells are induced to detach by trypsinor EDTA; or seen spontaneously during mitotic detachment. The overexpression of CDCP1 leads to the loss of cell adhesion and a detached phenotype.

Concentration:

Activity: Not validated for activity

Sequence: Phe30-Ser341

Purity: > 85 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 36.1 kDa

ObservedMW: 55 kDa