Recombinant Human Carbonic Anhydrase 1/CA1 Protein (His Tag)

SKU: PKSH032156

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: Carbonic Anhydrase 1;CA1

Target Synonym: CA-I;CA1;CAB;Carbonate Dehydratase I;Carbonic Anhydrase 1;Carbonic Anhydrase B;Carbonic Anhydrase I

Research Areas: Cell biology;Cardiovascular

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: C-His

UNIProt ID: P00915

Accession: P00915

Background: Carbonic Anhydrase 1 (CA1) is a cytosolic enzyme, belonging to the alpha-carbonic anhydrase family. It is highly expressed in erythrocytes and acts as an early marker for erythroid differentiation. Carbonic anhydrase 1 plays a improtant role in many biological processes such as calcification, cellular respiration, bone resorption, acid-base balance. It is activated by imidazole, histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. At the same time, It is inhibited by sulfonamide derivatives and coumarins. In addition, CA1 is a zinc metalloenzyme that has reversible hydration of carbon dioxide. It can hydrate cyanamide to urea.

Concentration:

Activity: Not validated for activity

Sequence: Ala2-Phe261

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 12.5mM Tris-HCl, 75mM NaCl, pH 7.5.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 29.9 kDa

ObservedMW: 25-35 kDa