Recombinant Human CA5A/CA-VA Protein (His Tag)

SKU: PKSH031568

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: CA5;CA5AD;CA5D;CAV;CAVA;Carbonic Anhydrase VA;GS1-21A4.1

Research Areas: Signal Transduction;Tags & Cell Markers;metabolism;

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Enzyme

Fusion tag: C-His

UNIProt ID: P35218

Accession: NP_001730.1

Background: Carbonic anhydrase 5A, mitochondrial, also known as Carbonate dehydratase VA, Carbonic anhydrase VA, CA-VA and CA5A, is a member of thealpha-carbonic anhydrase family. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA5A / CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt).

Concentration:

Activity: Measured by its esterase activity. The specific activity is > 500 pmoles/min/μg.

Sequence: Ala 40-Ser 305

Purity: > 96 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 50mM NaAc, 50mM NaCl, 0.05% Brij 35, pH 5.0
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: Please contact us for more information.

Calculated MW: 31.6 kDa

ObservedMW: 33 kDa