Recombinant Human Bcl-2 protein

SKU: CD02276

Size:5 ug

Background:B-cell lymphoma 2 (Bcl-2) is the founding member of the Bcl-2 family and it is encoded by the BCL2 gene in human. Bcl-2 forms homodimer and heterodimers with other Bcl-2 family proteins, like BAX, BAK, BAD and Bcl-xL. Alternative splicing of Bcl-2 results in two isoforms with similar folds despite differences in anti-apoptotic activity. Bcl-2 suppresses apoptosis by controlling the mitochondrial membrane permeability. It inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Antibodies to Bcl-2 can be used with immunohistochemistry to identify cells containing the antigen. In some cases, the presence or absence of Bcl-2 staining in biopsies may be significant for the patients prognosis or likelihood of cancer relapse. Mature human Bcl-2 shares 88 % - 90 % amino acid sequence identity with Mouse, rat, bovine, canine Bcl-2.

Research_area: Cell Biology

Purity:0

Source:0

Full product name:Recombinant Human B-cell Lymphoma 2

Biological activity:

Synonyms:Bcl-2,PPP1R5,BCL2, Human

Endotoxin:Less than 0.1 EU/μg of rHuBcl-2 as determined by LAL method.

Storage:-20ºC

Sequence:AHAGRTGYDN REIVMKYIHY KLSQRGYEWD AGDVGAAPPG AAPAPGIFSS QPGHTPHPAA SRDPVARTSP LQTPAAPGAA AGPALSPVPP VVHLTLRQAG DDFSRRYRRD FAEMSSQLHL TPFTARGRFA TVVEELFRDG VNWGRIVAFF EFGGVMCVES VNREMSPLVD NIALWMTEYL NRHLHTWIQD NGGWDAFVEL YGPSMRPLFD

uniprot accession:O6238

Molecular weight:Approximately 23.2 kDa, a single non-glycosylated polypeptide chain containing 21 amino acids.

Formulation:

Reconstitution:.2 μm filtered concentrated solution in 25 mM Tris-HCl, pH 8., 1mM NaCl, 1 mM DTT, 3 % Glycerol, with Tween-8.