Recombinant Human BCA-1/CXCL13 protein

SKU: CD02280

Size:5 ug

Background:C-X-C motif chemokine 13 encoded by the CXCL13 gene located on human chromosome 4 is constitutively expressed in secondary lymphoid organs. It is also known as B lymphocyte chemoattractant (BLC). As its name suggests, CXCL13 is selectively chemotactic for B cells belonging to both the B-1 and B-2 subsets, and elicits its effects by interacting with chemokine receptor CXCR5. It and its receptor CXCR5 control the organization of B cells within follicles of lymphoid tissues. Recombinant human CXCL13 is a single non-glycosylated polypeptide chain containing 87 amino acids and mature human BCA-1 shares 64 % amino acid sequence similarity with the Mouse protein and 23–34 % amino acid sequence identity with other known CXC chemokines.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human BCA-1/CXCL13

Biological activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human B cells is in a concentration range of 1.-1 ng/ml.

Synonyms:BCA-1/CXCL13,ANGIE, ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13, Human

Endotoxin:Less than 1 EU/ug of rHuBCA-1/CXCL13 as determined by LAL method.

Storage:-20ºC

Sequence:VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP

uniprot accession:O43927

Molecular weight:Approximately 1.3 kDa, a single non-glycosylated polypeptide chain containing 87 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM PB, pH 7.4, 1 mM NaCl.