Recombinant Human B-lymphocyte antigen CD20 (MS4A1)-Detergent (Active)

SKU: P11836

Size: 100ug. Other sizes are also available.

Activity: Yes

Research Areas: Cancer

Uniprot ID: P11836

Gene Names: MS4A1

Alternative Name(s): (B-lymphocyte surface antigen B1) (Bp35) (Leukocyte surface antigen Leu-16) (Membrane-spanning 4-domains subfamily A member 1) (CD20)

Abbreviation: Recombinant Human MS4A1 protein-Detergent (Active)

Organism: Homo sapiens (Human)

Source: Mammalian cell

Expression Region: 1-297aa

Protein Length: Full Length

Tag Info: C-terminal 10xHis-tagged

Target Protein Sequence: MTTPRNSVNGTFPAEPMKGPIAMQSGPKPLFRRMSSLVGPTQSFFMRESKTLGAVQIMNGLFHIALGGLLMIPAGIYAPICVTVWYPLWGGIMYIISGSLLAATEKNSRKCLVKGKMIMNSLSLFAAISGMILSIMDILNIKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP

MW: 34.4 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD20 at 2 μg/mL on an Nickel Coated plate can bind Anti-CD20 recombinant antibody (CSB-RA015007A1HU), the EC50 is 4.034-6.968 ng/mL.Measured by its binding ability in a functional ELISA. Immobilized Human CD20 at 2 μg/mL on an Nickel Coated plate can bind Anti-CD20 recombinant antibody (CSB-RA015007MA3HU), the EC50 is 8.137-9.704 ng/mL.

Form: Liquid

Buffer: 50 mM HEPES, 0.15 M NaCl, 0.05% DDM, 0.01% CHS, pH7.5

Reconstitution: /

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed: 3925015, PubMed: 7684739, PubMed: 12920111). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed: 7684739, PubMed: 12920111, PubMed: 18474602).

Reference: "CD20 homo-oligomers physically associate with the B cell antigen receptor. Dissociation upon receptor engagement and recruitment of phosphoproteins and calmodulin-binding proteins." Polyak M.J., Li H., Shariat N., Deans J.P. J. Biol. Chem. 283: 18545-18552(2008)

Function: