Recombinant Human Autotaxin/ENPP2 Protein (aa 36-863, His Tag)

SKU: PKSH033693

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: Autotaxin;ENPP2

Target Synonym: ATX;ATX-X;ATXFLJ26803;Autotaxin;E-NPP 2;EC 3.1.4.39;ENPP2;LysoPLD;NPP2;PD-IALPHA;PDNP2;PDNP2NPP2;autotaxin-t;ectonucleotide pyrophosphatase/phosphodiesterase 2

Research Areas: Signal Transduction;Cardiovascular;Neuroscience;Cancer;metabolism;

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: Q13822

Accession: AAH34961.1

Background: ENPP-2, also known as Autotaxin, belongs to the ectonucleotide pyrophosphatase/phosphodiesterase (NPP) family. Some NPPs hydrolyze phosphates from nucleotides and their derivatives. ENPP-2 shares 40 - 50% identity to ENPP1 & 3, all of which contain a N-terminal intracellular domain, a single transmembrane domain and a large extracellular domain that includes a catalytic domain, two somatomedin-B-like domains, and a C-terminal nuclease-like domain.Evidence shows LPA and sphingosine 1-phosphate to be specific inhibitors of ENPP-2. ENPP-2 was originally found to stimulate tumor cell motility and has since been found to enhance tumor invasion and metastasis and to be up-regulated in several types of carcinomas including breast and lung.

Concentration:

Activity: Not validated for activity

Sequence: Ala36-Ile863

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mMPB,150mMNaCl,pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 96 kDa

ObservedMW: 100-120 kDa