Recombinant Human 14-3-3 sigma/YWHAS Protein (GST Tag)

SKU: PKSH031401

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: 14-3-3 Protein Sigma;Epithelial Cell Marker Protein 1;HME1;SFN;Stratifin

Research Areas: Signal Transduction;Neuroscience;Stem cells

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: N-GST

UNIProt ID: P31947

Accession: NP_006133.1

Background: 14-3-3 protein sigma (YWHAS); also known as stratifin (SFN) and epithelial cell marker protein 1; is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases; phosphatases; and transmembrane receptors; and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2; and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally; YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.

Concentration:

Activity: Not validated for activity

Sequence: Met 1-Ser 248

Purity: > 94 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20mM Tris, 150mM NaCl, 10mM GSH, 25% glycerol, pH 8.0
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: Please contact us for more information.

Calculated MW: 50.1 kDa

ObservedMW: 48 kDa