Recombinant Histone H3 (Symmetric Di Methyl Arg2) Monoclonal Antibody

SKU: AN302110L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies;Acetyl/Phospho/Methyl Antibodies

Abbreviation:Histone H3 (Symmetric Di Methyl Arg2)

Target Synonym:Histone H;H3C;H3/A;H3C2;H3C3;H3C4;H3C6;H3C7;H3C8;H3FA;H3C10;H3C11;H3C12;HIST1H3A;H3C1;Histone H3.1;Histone H3/a;Histone H3/b;H3FL;HIST1H3B;H3FC;HIST1H3C;H3FB;HIST1H3D;H3FD;HIST1H3E;H3FI;HIST1H3F;H3FH;HIST1H3G;H3FK;HIST1H3H;H3FF;HIST1H3I;H3FJ;HIST1H3J;H3/A;H3FA;H3FB;H3FC;H3FD;H3FF;H3FH;H3FI;H3FJ;H3FK;H3FL;Hist1h3a;HIST1H3B;HIST1H3C;HIST1H3D;HIST1H3E;HIST1H3F;HIST1H3G;HIST1H3H;HIST1H3I;HIST1H3J;Histone H3.1;Histone H3/a;Histone H3/b;H3 histone family;H31;H3a;histone 1;Histone cluster 1;Histone H3/c;Histone H3/d;Histone H3/f;Histone H3/h;Histone H3/i;Histone H3/j;Histone H3/k;Histone H3/l;member A

Research Areas:Epigenetics and Nuclear Signaling;Isotype;Loading Controls

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Rat;Mouse

Application:WB;ChIP

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A834

UNIProt ID:P68431

Accession:

Background:The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine methylation in multiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation in core histones is a major determinant for the formation of active and inactive regions of the genome and therefore plays vital roles in multiple cellular events. In most species, lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing. Methylation in histones modulated by specific histone methyltransferases (HMTs) and histone demethylases (HDMs) is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine methylation have become promising targets for anti-cancer drugs.

Concentration:1 mg/mL

Immunogen:Recombinant human Symmetric Di-Methyl-Histone H3 (Arg2) fragment

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:1000-1:2000;ChIP 6 μg/5×10⁶ cells

Calculated MW:15 kDa

ObservedMW:15 kDa