Recombinant Histone H3.3 Monoclonal Antibody

SKU: AN302024L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:Histone H3.3

Target Synonym:H3F;histone H;H3F3A;H3.3A;H3F3;histone H3.3;family 3B (H3.3B);H3 histone;H3 histone family 3A;H3 histone family 3B;H3.3;H33;Histone H3.3Q;Histone H3.A;Histone H3.B;MGC87782;MGC87783;H3.3B;H3F3B;H3-3A;H3-3B;PP781

Research Areas:Epigenetics and Nuclear Signaling

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Rat;Mouse

Application:WB;IHC

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A744

UNIProt ID:P84243

Accession:

Background:Histone H3.3 is a histone H3 variant that replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Concentration:1 mg/mL

Immunogen:Peptide. This information is proprietary to PTMab.

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:1000;IHC 1:500-1:1000

Calculated MW:15 kDa

ObservedMW:15 kDa