Recombinant Histone H2B (Acetyl Lys46) Monoclonal Antibody

SKU: AN301406L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies;Acetyl/Phospho/Methyl Antibodies

Abbreviation:Histone H2B (Acetyl Lys46)

Target Synonym:HIST1H2BG;H2B.1A;H2B/a;H2BFA;H2BFL;HIST1H2BC;H2BFH;HIST1H2BE;H2BFG;HIST1H2BF;H2BFK;HIST1H2BI;H2BC4;H2BC6;H2BC7;H2BC8;H2BC10

Research Areas:Epigenetics and Nuclear Signaling

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Rat;Mouse

Application:WB;ChIP;IF

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A101

UNIProt ID:P62807

Accession:

Background:Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone acetylation occurs primarily at multiple lysine residues on the amino-terminal of core histones, in response to various stimuli and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Mostly, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15, 16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are major regulating factors.

Concentration:1 mg/mL

Immunogen:Acetylated human histone H2B (Lys46) peptide

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:500-1:2000;ChIP 6 μg/5×10⁶ cells;IF 1:200

Calculated MW:14 kDa

ObservedMW:15kDa