Recombinant Heteroscodra maculata Kappa-theraphotoxin-Hm1a

CSB-EP351577HGUa3

Size: 200ug. Other sizes are also available. Please Inquire.

In Stock: No

Lead time: 10-20 working days

Research Topic: Others

Uniprot ID: P60992

Gene Names: N/A

Organism: Heteroscodra maculata (Togo starburst tarantula）(Togo starburst baboon spider)

AA Sequence: ECRYLFGGCSSTSDCCKHLSCRSDWKYCAWDGTFS

Expression Region: 1-35aa

Sequence Info: Full Length

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged and C-terminal Myc-tagged

MW: 21.5 kDa

Alternative Name(s): Heteroscodratoxin-1

Relevance: Gating modifier toxin that principally inhibits inactivation of the mammalian voltage-gated sodium channel Nav1.1/SCN1A. When tested against human Nav1.1/SCN1A-Nav1.8/SCN10A alpha subunits heterologously expressed in Xenopus oocytes, the toxin inhibits inactivation of Nav1.1 (EC(50)=38 nM), with substantially weaker effects on Nav1.2/SCN2A (EC(50)=236 nM) and Nav1.3/SCN3A (EC(50)=220 nM). The toxin inhibits several subtypes of voltage-gated potassium channels with significantly lower potency than its effects on Nav1.1/SCN1A. It is a moderately potent blocker of Kv2.1/KCNB1, Kv2.2/KCNB2, Kv4.1/KCND1, Kv4.2/KCND2 and Kv4.3/KCND3 and has little effect on Kv1.1/KCNA1, Kv1.2/KCNA2, Kv1.3/KCNA3, Kv1.4/KCNA4, Kv1.5/KCNA5, Kv1.6/KCNA6 and Kv3.4/KCNC4. The binding affinity and subtype selectivity of the toxin is determined by residues within both the S1-S2 and S3-S4 loops of the domain IV voltage sensor. Intracerebroventricular injection into mice elicits convulsions, spasms, tremors and rapid death. When injected into mouse hindpaw, the toxin elicits an immediate and robust response to pain. Intraplantar injection of toxin does not cause neurogenic inflammation or alter sensitivity to heat, indicative of a modality-specific effect on mechanosensitive neurons

Reference: "Novel tarantula toxins for subtypes of voltage-dependent potassium channels in the Kv2 and Kv4 subfamilies." Escoubas P., Diochot S., Celerier M.-L., Nakajima T., Lazdunski M. Mol. Pharmacol. 62:48-57(2002)

Purity: Greater than 85% as determined by SDS-PAGE.

Storage Buffer: Tris-based buffer，50% glycerol

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.