Recombinant HCV (HCV-1a) NS3 protease / helicase immunodominant region Protein (aa 1356-1459, GST Tag)

SKU: PKSV030179

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Viral Proteins

Abbreviation:

Target Synonym: HCV;NS3 Protein

Research Areas:

Conjugation:

Target Species: HCV

Expression Host: E.coli

Application:

Fusion tag: N-GST

UNIProt ID:

Accession: NP_803144.1

Background: HCV NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. HCV NS3 serine protease, in association with NS4A, is responsible for the cleavages of NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS3/NS4A complex also prevents phosphorylation of human IRF3, thus preventing the establishment of dsRNA induced antiviral state. HCV NS3 RNA helicase binds to RNA and unwinds dsRNA in the 3' to 5' direction, and likely RNA stable secondary structure in the template strand (By similarity). Cleaves and inhibits the host antiviral protein MAVS.

Concentration:

Activity: Not validated for activity

Sequence: Thr1356-Thr1459

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 50 mM Tris, 500 mM NaCl.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: Please contact us for more information.

Calculated MW: 37.9 kDa

ObservedMW: