Recombinant Cyclin D3 Monoclonal Antibody

SKU: AN301504L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:Cyclin D3

Target Synonym:G1 S specific cyclin D;G1/S specific cyclin D;G1/S-specific cyclin-D;CyclinD;cyclin D;CCND3;cyclin D3;CCND 3;CyclinD3;D3 type cyclin;G1 S specific cyclin D3;G1/S specific cyclin D3;G1/S-specific cyclin-D3

Research Areas:Cell Biology;Epigenetics and Nuclear Signaling;Cancer

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;

Application:WB;IHC

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A203

UNIProt ID:P30281

Accession:

Background:Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Concentration:1 mg/mL

Immunogen:Recombinant human Cyclin D3 fragment

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:500-1:2000;IHC 1:50-1:100

Calculated MW:33 kDa

ObservedMW:33 kDa