Recombinant Chicken Neurofibromin (NF1), partial

SKU: P35608

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Others

Uniprot ID: P35608

Gene Names: NF1

Alternative Name(s): (Neurofibromatosis-related protein NF-1)

Abbreviation: Recombinant Chicken NF1 protein, partial

Organism: Gallus gallus (Chicken)

Source: E.coli

Expression Region: 1-270aa

Protein Length: Partial

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Target Protein Sequence: RARVIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALTSLETVTEALLEIMEACMRDIPTCKWLDQWTELAQKFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKDSPMHKALFWVAMAVLELDEVNLYSAGTALLEQNLHTLDSLRVFNDKSPEEVFMEIRRPLEWHCKQMDHFVGLNFNSNFNFALVGPGP

MW: 35.8 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity.

Reference: "Analysis of the sequence and embryonic expression of chicken neurofibromin mRNA." Schafer G.L., Ciment G., Stocker K.M., Baizer L. Mol. Chem. Neuropathol. 18: 267-278(1993)

Function: