Recombinant Bat coronavirus 133/2005 Nucleoprotein (N)

SKU: Q0Q4E6

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Microbiology

Uniprot ID: Q0Q4E6

Gene Names: N

Alternative Name(s): Nucleocapsid protein;NC;Protein N

Abbreviation: Recombinant Bat coronavirus 133/2005 N protein

Organism: Bat coronavirus 133/2005 (BtCoV) (BtCoV/133/2005)

Source: Baculovirus

Expression Region: 1-424aa

Protein Length: Full Length

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Target Protein Sequence: MATPAAPRTISFADNNDNQPNQQQRGRGRNPKPRPAPNNTVSWYTGLTQHGKNPLAFPPGQGVPLNANSTTAQNAGYWRRQDRKINTGNGVKQLAPRWFFYYTGTGPEANLPFRSVKDGIVWVYEEGATDAPSVFGTRNPANDAAIVCQFAPGTLIPKNFHIEGTGGNSQSSSRASSNSRNSSRSNSRGGRSTSNSRGTSPVSHGVGSAESLAALPLLLDLQKRLADLESGKSKQPKVVTKKDAAAAKNKMRHKRVATKGFNVTQAFGLRGPGPLQGNFGDMNYNKFGTEDPRWPQMAELAPSASAFMSMSQFKLTHQSNDDKGDPIYFLSYSGAIKLDPKNPNYKKWLELLEANIDAYKTFPKKERKPKTTEDGAVVASSSASQMEDVDAKPQRKPKSRVAGSITMRSGSSPALQDVTFDSEA

MW: 49.9 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.

Reference:

Function: