Rat Steroid 17-alpha-hydroxylase, CYP17A1 ELISA Kit

SKU: E2264Ra

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis. Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol. Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione. Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA. Also 16-alpha hydroxylates androgens, relevant for estriol synthesis. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).

Research_area:Cardiovascular

Applications:ELISA

Target_protein:CYP17A1

Reactivity:Rat

Assay type:Sandwich

Sensitivity:7.75ng/L

Detection range:15-3000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Steroid 17-alpha-hydroxylase/17,20 lyase

Species:Rat

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P11715

MASS(Da):57,250

GeneID:25146

Synonyms:17-alpha-hydroxyprogesterone aldolase;Cyp17;CYP17A1;CYPXVII;Cytochrome P450 17A1;Cytochrome P450c17;Cytochrome P450-C17;Steroid 17-alpha-hydroxylase/17,20 lyase;Steroid 17-alpha-monooxygenase;

Gene names:Cyp17a1

Standard solution:3200ng/L